Response factor

Response factor

concentration of µg/ ml is made and results in a peak area of Calculate the response factor for benzene using Equation 1. response factor = = 50.
Wish to use the response factor in determining the purity of my compound.
A “ response factor ” must be calculated for each substance with a particular detector. A response factor is obtained experimentally by analyzing. Gene Regulation Meeting Reports and Events. If the curve is linear then you can use the average of the response factors to calculate the conc. Click here for advertising rates! Click here to cancel reply. One of the main reasons to use response factors is to compensate for the irreproducibility of manual injections into a gas chromatograph GC. However, this would assume that the two compounds have identical UV absorption at the analysis wavelength. These equations can be Response factor to determine analyte concentration over a linear concentration range using chromatographic methods e. Response factor

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After analysis, the results required are the peak areas of A, B and IS. This way if the injection volumes and hence the peak areas differ slightly, the ratio of the areas of the analyte and the internal standard will remain constant from one run to the next. It depends on the experiment. You must know the nature of the compounds impurities you wish to estimate. Click here for advertising rates!